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endogenous parp1 protein  (Proteintech)


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    Proteintech endogenous parp1 protein
    Area of irradiated and unirradiated <t>HeLa-Parp1</t> – CB-tagRFP cells. Cells on the left side of the dotted blue line were irradiated with 10 or 100 Cpp (orange and red target points, respectively) and allowed to recover for 1.5 h. For targeting, a single slice image of the Parp1 signal of the cells was taken (orange frame). Neighbouring cells on the right side of the blue dotted line were not irradiated and were used as controls. After 5EU incorporation and immunofluorescence staining, cells were imaged as z -stacks of 33 layers, with 300 nm distance in the single channels of Parp1, 5EU, γH2AX and phase contrast. Stacks were deconvolved for improved depth resolution. A single slice from the centre of the stack is shown. Arrows mark the target and hit areas verified by γH2AX. The best examples are the hit nucleoli in the three bottom cells (marked by asterisks). The outlines of these nucleoli in the phase contrast image still correlate with the outline before irradiation. The γH2AX foci are completely embedded in the nucleoli and at their sites; 5EU and Parp1 signals are less intense as in direct proximity. However, the irradiated nucleoli and the residual nucleoli of the corresponding nucleus do not show an overall decrease in 5EU signal. Fluctuations in brightness correlate with Parp1 fluctuations before irradiation and are also observable in control cells.
    Endogenous Parp1 Protein, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/endogenous+parp1+protein/PARP1+Fusion+Protein/pmc06803363-215-7-4
    Average 95 stars, based on 39 article reviews
    endogenous parp1 protein - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Local inhibition of rRNA transcription without nucleolar segregation after targeted ion irradiation of the nucleolus"

    Article Title: Local inhibition of rRNA transcription without nucleolar segregation after targeted ion irradiation of the nucleolus

    Journal: Journal of Cell Science

    doi: 10.1242/jcs.232181

    Area of irradiated and unirradiated HeLa-Parp1 – CB-tagRFP cells. Cells on the left side of the dotted blue line were irradiated with 10 or 100 Cpp (orange and red target points, respectively) and allowed to recover for 1.5 h. For targeting, a single slice image of the Parp1 signal of the cells was taken (orange frame). Neighbouring cells on the right side of the blue dotted line were not irradiated and were used as controls. After 5EU incorporation and immunofluorescence staining, cells were imaged as z -stacks of 33 layers, with 300 nm distance in the single channels of Parp1, 5EU, γH2AX and phase contrast. Stacks were deconvolved for improved depth resolution. A single slice from the centre of the stack is shown. Arrows mark the target and hit areas verified by γH2AX. The best examples are the hit nucleoli in the three bottom cells (marked by asterisks). The outlines of these nucleoli in the phase contrast image still correlate with the outline before irradiation. The γH2AX foci are completely embedded in the nucleoli and at their sites; 5EU and Parp1 signals are less intense as in direct proximity. However, the irradiated nucleoli and the residual nucleoli of the corresponding nucleus do not show an overall decrease in 5EU signal. Fluctuations in brightness correlate with Parp1 fluctuations before irradiation and are also observable in control cells.
    Figure Legend Snippet: Area of irradiated and unirradiated HeLa-Parp1 – CB-tagRFP cells. Cells on the left side of the dotted blue line were irradiated with 10 or 100 Cpp (orange and red target points, respectively) and allowed to recover for 1.5 h. For targeting, a single slice image of the Parp1 signal of the cells was taken (orange frame). Neighbouring cells on the right side of the blue dotted line were not irradiated and were used as controls. After 5EU incorporation and immunofluorescence staining, cells were imaged as z -stacks of 33 layers, with 300 nm distance in the single channels of Parp1, 5EU, γH2AX and phase contrast. Stacks were deconvolved for improved depth resolution. A single slice from the centre of the stack is shown. Arrows mark the target and hit areas verified by γH2AX. The best examples are the hit nucleoli in the three bottom cells (marked by asterisks). The outlines of these nucleoli in the phase contrast image still correlate with the outline before irradiation. The γH2AX foci are completely embedded in the nucleoli and at their sites; 5EU and Parp1 signals are less intense as in direct proximity. However, the irradiated nucleoli and the residual nucleoli of the corresponding nucleus do not show an overall decrease in 5EU signal. Fluctuations in brightness correlate with Parp1 fluctuations before irradiation and are also observable in control cells.

    Techniques Used: Irradiation, Immunofluorescence, Staining, Control

    Certain nucleoli hits after irradiation with 1, 10 and 50 Cpp and their influence on rRNA transcription. Recovery time was between 30 min and 7 h. Target definition images before irradiation are shown on the left. γH2AX, 5EU, Parp1 and phase contrast images of single cells were taken in the focus depth of the γH2AX foci after IF staining. Additionally, intensity line plots through the γH2AX foci along the white dotted line of the merged γH2AX+5EU image are shown on the right. Contrast was chosen such that 0.1% of the pixels were in saturation. The intensity plots show how 5EU and Parp1 signals (red and blue, respectively) were locally reduced at the damage site marked by γH2AX (black). Two-dimensional location is marked by yellow arrows. In these images, this correlates with local reduction of Parp1 and at higher doses additionally with altered appearance of the nucleolus in the phase contrast image. For better cell reconstruction, we used tiled images that consisted of 2×2 fields of view from the camera before irradiation and 3×3 after IF staining; thus, some cell images show the edge of the tiles. Scale bars: 3 µm.
    Figure Legend Snippet: Certain nucleoli hits after irradiation with 1, 10 and 50 Cpp and their influence on rRNA transcription. Recovery time was between 30 min and 7 h. Target definition images before irradiation are shown on the left. γH2AX, 5EU, Parp1 and phase contrast images of single cells were taken in the focus depth of the γH2AX foci after IF staining. Additionally, intensity line plots through the γH2AX foci along the white dotted line of the merged γH2AX+5EU image are shown on the right. Contrast was chosen such that 0.1% of the pixels were in saturation. The intensity plots show how 5EU and Parp1 signals (red and blue, respectively) were locally reduced at the damage site marked by γH2AX (black). Two-dimensional location is marked by yellow arrows. In these images, this correlates with local reduction of Parp1 and at higher doses additionally with altered appearance of the nucleolus in the phase contrast image. For better cell reconstruction, we used tiled images that consisted of 2×2 fields of view from the camera before irradiation and 3×3 after IF staining; thus, some cell images show the edge of the tiles. Scale bars: 3 µm.

    Techniques Used: Irradiation, Staining

    Cross-sections through z -stacks of certain nucleolus hit examples used for 5EU and Parp1 analysis. (A) 5EU and Parp1 signals were analysed in the central slice of the γH2AX foci (compare with <xref ref-type=Fig. 2 ). Nucleolar signal of 5EU is in green and γH2AX in red. The z -stacks shown consist of 13 slices with distance 300 nm, containing information of the single nucleus. (B) Analysis of single foci showing certain nucleolus hits on 5EU incorporation (left) and Parp1 distribution (right). Boxplots show median of the 5EU and Parp1 intensity ( I 5EU , I Parp1 ) ratios, in which the box represents the 2nd quartile, error bars show 1.5× the interquartile range and dots indicate outliers of the distribution. The signal intensity at the focus I …,focus minus the nucleus background signal I …,nucleus was calculated as a ratio of the signal intensity in the residual nucleolus I …,nucleolus minus the nucleus background signal I …,nucleus . 5EU and Parp1 signals were significantly reduced at the DNA damage site marked by γH2AX. A one-sample signed rank test (*) was used to test the hypothesis H 0 : median=1, which gave P <0.001. For the higher rates of 10 and 50 Cpp, 5EU incorporation and Parp1 intensity significantly decreased to about 0.2 and 0.5, respectively. The Mann–Whitney rank sum test was used to test the difference of the medians for 10 or 50 Cpp compared with 1 Cpp, which gave + P <0.001 for 5EU and ++ P <0.05 for Parp1. Scale bars: 3 µm (for all cross-sections). " title="... certain nucleolus hit examples used for 5EU and Parp1 analysis. (A) 5EU and Parp1 signals were analysed ..." property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Cross-sections through z -stacks of certain nucleolus hit examples used for 5EU and Parp1 analysis. (A) 5EU and Parp1 signals were analysed in the central slice of the γH2AX foci (compare with Fig. 2 ). Nucleolar signal of 5EU is in green and γH2AX in red. The z -stacks shown consist of 13 slices with distance 300 nm, containing information of the single nucleus. (B) Analysis of single foci showing certain nucleolus hits on 5EU incorporation (left) and Parp1 distribution (right). Boxplots show median of the 5EU and Parp1 intensity ( I 5EU , I Parp1 ) ratios, in which the box represents the 2nd quartile, error bars show 1.5× the interquartile range and dots indicate outliers of the distribution. The signal intensity at the focus I …,focus minus the nucleus background signal I …,nucleus was calculated as a ratio of the signal intensity in the residual nucleolus I …,nucleolus minus the nucleus background signal I …,nucleus . 5EU and Parp1 signals were significantly reduced at the DNA damage site marked by γH2AX. A one-sample signed rank test (*) was used to test the hypothesis H 0 : median=1, which gave P <0.001. For the higher rates of 10 and 50 Cpp, 5EU incorporation and Parp1 intensity significantly decreased to about 0.2 and 0.5, respectively. The Mann–Whitney rank sum test was used to test the difference of the medians for 10 or 50 Cpp compared with 1 Cpp, which gave + P <0.001 for 5EU and ++ P <0.05 for Parp1. Scale bars: 3 µm (for all cross-sections).

    Techniques Used: MANN-WHITNEY

    Analysis of single foci showing certain nucleolus hits on 5EU incorporation and Parp1 distribution. Frequency of single foci, showing certain nucleolus hits with dips in the 5EU and Parp1 signals. If the ratio was below 0.80, the area was defined as a dip. Error bars describe the 95% confidence Wilson score intervals of the binomial proportion.
    Figure Legend Snippet: Analysis of single foci showing certain nucleolus hits on 5EU incorporation and Parp1 distribution. Frequency of single foci, showing certain nucleolus hits with dips in the 5EU and Parp1 signals. If the ratio was below 0.80, the area was defined as a dip. Error bars describe the 95% confidence Wilson score intervals of the binomial proportion.

    Techniques Used:

    Related Articles

    Irradiation:

    Article Title: Local inhibition of rRNA transcription without nucleolar segregation after targeted ion irradiation of the nucleolus
    Article Snippet: A fluorescent, tagRFP-labelled chromobody (ChromoTek) marks the endogenous Parp1 protein in these cells.

    Immunofluorescence:

    Article Title: Local inhibition of rRNA transcription without nucleolar segregation after targeted ion irradiation of the nucleolus
    Article Snippet: A fluorescent, tagRFP-labelled chromobody (ChromoTek) marks the endogenous Parp1 protein in these cells.

    Staining:

    Article Title: Local inhibition of rRNA transcription without nucleolar segregation after targeted ion irradiation of the nucleolus
    Article Snippet: A fluorescent, tagRFP-labelled chromobody (ChromoTek) marks the endogenous Parp1 protein in these cells.

    Control:

    Article Title: Local inhibition of rRNA transcription without nucleolar segregation after targeted ion irradiation of the nucleolus
    Article Snippet: A fluorescent, tagRFP-labelled chromobody (ChromoTek) marks the endogenous Parp1 protein in these cells.

    MANN-WHITNEY:

    Article Title: Local inhibition of rRNA transcription without nucleolar segregation after targeted ion irradiation of the nucleolus
    Article Snippet: A fluorescent, tagRFP-labelled chromobody (ChromoTek) marks the endogenous Parp1 protein in these cells.



    Similar Products

    endogenous parp1 protein  (Proteintech)
    95
    Proteintech endogenous parp1 protein
    Area of irradiated and unirradiated <t>HeLa-Parp1</t> – CB-tagRFP cells. Cells on the left side of the dotted blue line were irradiated with 10 or 100 Cpp (orange and red target points, respectively) and allowed to recover for 1.5 h. For targeting, a single slice image of the Parp1 signal of the cells was taken (orange frame). Neighbouring cells on the right side of the blue dotted line were not irradiated and were used as controls. After 5EU incorporation and immunofluorescence staining, cells were imaged as z -stacks of 33 layers, with 300 nm distance in the single channels of Parp1, 5EU, γH2AX and phase contrast. Stacks were deconvolved for improved depth resolution. A single slice from the centre of the stack is shown. Arrows mark the target and hit areas verified by γH2AX. The best examples are the hit nucleoli in the three bottom cells (marked by asterisks). The outlines of these nucleoli in the phase contrast image still correlate with the outline before irradiation. The γH2AX foci are completely embedded in the nucleoli and at their sites; 5EU and Parp1 signals are less intense as in direct proximity. However, the irradiated nucleoli and the residual nucleoli of the corresponding nucleus do not show an overall decrease in 5EU signal. Fluctuations in brightness correlate with Parp1 fluctuations before irradiation and are also observable in control cells.
    Endogenous Parp1 Protein, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/endogenous+parp1+protein/PARP1+Fusion+Protein/pmc06803363-215-7-4
    Average 95 stars, based on 1 article reviews
    endogenous parp1 protein - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    Area of irradiated and unirradiated HeLa-Parp1 – CB-tagRFP cells. Cells on the left side of the dotted blue line were irradiated with 10 or 100 Cpp (orange and red target points, respectively) and allowed to recover for 1.5 h. For targeting, a single slice image of the Parp1 signal of the cells was taken (orange frame). Neighbouring cells on the right side of the blue dotted line were not irradiated and were used as controls. After 5EU incorporation and immunofluorescence staining, cells were imaged as z -stacks of 33 layers, with 300 nm distance in the single channels of Parp1, 5EU, γH2AX and phase contrast. Stacks were deconvolved for improved depth resolution. A single slice from the centre of the stack is shown. Arrows mark the target and hit areas verified by γH2AX. The best examples are the hit nucleoli in the three bottom cells (marked by asterisks). The outlines of these nucleoli in the phase contrast image still correlate with the outline before irradiation. The γH2AX foci are completely embedded in the nucleoli and at their sites; 5EU and Parp1 signals are less intense as in direct proximity. However, the irradiated nucleoli and the residual nucleoli of the corresponding nucleus do not show an overall decrease in 5EU signal. Fluctuations in brightness correlate with Parp1 fluctuations before irradiation and are also observable in control cells.

    Journal: Journal of Cell Science

    Article Title: Local inhibition of rRNA transcription without nucleolar segregation after targeted ion irradiation of the nucleolus

    doi: 10.1242/jcs.232181

    Figure Lengend Snippet: Area of irradiated and unirradiated HeLa-Parp1 – CB-tagRFP cells. Cells on the left side of the dotted blue line were irradiated with 10 or 100 Cpp (orange and red target points, respectively) and allowed to recover for 1.5 h. For targeting, a single slice image of the Parp1 signal of the cells was taken (orange frame). Neighbouring cells on the right side of the blue dotted line were not irradiated and were used as controls. After 5EU incorporation and immunofluorescence staining, cells were imaged as z -stacks of 33 layers, with 300 nm distance in the single channels of Parp1, 5EU, γH2AX and phase contrast. Stacks were deconvolved for improved depth resolution. A single slice from the centre of the stack is shown. Arrows mark the target and hit areas verified by γH2AX. The best examples are the hit nucleoli in the three bottom cells (marked by asterisks). The outlines of these nucleoli in the phase contrast image still correlate with the outline before irradiation. The γH2AX foci are completely embedded in the nucleoli and at their sites; 5EU and Parp1 signals are less intense as in direct proximity. However, the irradiated nucleoli and the residual nucleoli of the corresponding nucleus do not show an overall decrease in 5EU signal. Fluctuations in brightness correlate with Parp1 fluctuations before irradiation and are also observable in control cells.

    Article Snippet: A fluorescent, tagRFP-labelled chromobody (ChromoTek) marks the endogenous Parp1 protein in these cells.

    Techniques: Irradiation, Immunofluorescence, Staining, Control

    Certain nucleoli hits after irradiation with 1, 10 and 50 Cpp and their influence on rRNA transcription. Recovery time was between 30 min and 7 h. Target definition images before irradiation are shown on the left. γH2AX, 5EU, Parp1 and phase contrast images of single cells were taken in the focus depth of the γH2AX foci after IF staining. Additionally, intensity line plots through the γH2AX foci along the white dotted line of the merged γH2AX+5EU image are shown on the right. Contrast was chosen such that 0.1% of the pixels were in saturation. The intensity plots show how 5EU and Parp1 signals (red and blue, respectively) were locally reduced at the damage site marked by γH2AX (black). Two-dimensional location is marked by yellow arrows. In these images, this correlates with local reduction of Parp1 and at higher doses additionally with altered appearance of the nucleolus in the phase contrast image. For better cell reconstruction, we used tiled images that consisted of 2×2 fields of view from the camera before irradiation and 3×3 after IF staining; thus, some cell images show the edge of the tiles. Scale bars: 3 µm.

    Journal: Journal of Cell Science

    Article Title: Local inhibition of rRNA transcription without nucleolar segregation after targeted ion irradiation of the nucleolus

    doi: 10.1242/jcs.232181

    Figure Lengend Snippet: Certain nucleoli hits after irradiation with 1, 10 and 50 Cpp and their influence on rRNA transcription. Recovery time was between 30 min and 7 h. Target definition images before irradiation are shown on the left. γH2AX, 5EU, Parp1 and phase contrast images of single cells were taken in the focus depth of the γH2AX foci after IF staining. Additionally, intensity line plots through the γH2AX foci along the white dotted line of the merged γH2AX+5EU image are shown on the right. Contrast was chosen such that 0.1% of the pixels were in saturation. The intensity plots show how 5EU and Parp1 signals (red and blue, respectively) were locally reduced at the damage site marked by γH2AX (black). Two-dimensional location is marked by yellow arrows. In these images, this correlates with local reduction of Parp1 and at higher doses additionally with altered appearance of the nucleolus in the phase contrast image. For better cell reconstruction, we used tiled images that consisted of 2×2 fields of view from the camera before irradiation and 3×3 after IF staining; thus, some cell images show the edge of the tiles. Scale bars: 3 µm.

    Article Snippet: A fluorescent, tagRFP-labelled chromobody (ChromoTek) marks the endogenous Parp1 protein in these cells.

    Techniques: Irradiation, Staining

    Cross-sections through z -stacks of certain nucleolus hit examples used for 5EU and Parp1 analysis. (A) 5EU and Parp1 signals were analysed in the central slice of the γH2AX foci (compare with <xref ref-type=Fig. 2 ). Nucleolar signal of 5EU is in green and γH2AX in red. The z -stacks shown consist of 13 slices with distance 300 nm, containing information of the single nucleus. (B) Analysis of single foci showing certain nucleolus hits on 5EU incorporation (left) and Parp1 distribution (right). Boxplots show median of the 5EU and Parp1 intensity ( I 5EU , I Parp1 ) ratios, in which the box represents the 2nd quartile, error bars show 1.5× the interquartile range and dots indicate outliers of the distribution. The signal intensity at the focus I …,focus minus the nucleus background signal I …,nucleus was calculated as a ratio of the signal intensity in the residual nucleolus I …,nucleolus minus the nucleus background signal I …,nucleus . 5EU and Parp1 signals were significantly reduced at the DNA damage site marked by γH2AX. A one-sample signed rank test (*) was used to test the hypothesis H 0 : median=1, which gave P <0.001. For the higher rates of 10 and 50 Cpp, 5EU incorporation and Parp1 intensity significantly decreased to about 0.2 and 0.5, respectively. The Mann–Whitney rank sum test was used to test the difference of the medians for 10 or 50 Cpp compared with 1 Cpp, which gave + P <0.001 for 5EU and ++ P <0.05 for Parp1. Scale bars: 3 µm (for all cross-sections). " width="100%" height="100%">

    Journal: Journal of Cell Science

    Article Title: Local inhibition of rRNA transcription without nucleolar segregation after targeted ion irradiation of the nucleolus

    doi: 10.1242/jcs.232181

    Figure Lengend Snippet: Cross-sections through z -stacks of certain nucleolus hit examples used for 5EU and Parp1 analysis. (A) 5EU and Parp1 signals were analysed in the central slice of the γH2AX foci (compare with Fig. 2 ). Nucleolar signal of 5EU is in green and γH2AX in red. The z -stacks shown consist of 13 slices with distance 300 nm, containing information of the single nucleus. (B) Analysis of single foci showing certain nucleolus hits on 5EU incorporation (left) and Parp1 distribution (right). Boxplots show median of the 5EU and Parp1 intensity ( I 5EU , I Parp1 ) ratios, in which the box represents the 2nd quartile, error bars show 1.5× the interquartile range and dots indicate outliers of the distribution. The signal intensity at the focus I …,focus minus the nucleus background signal I …,nucleus was calculated as a ratio of the signal intensity in the residual nucleolus I …,nucleolus minus the nucleus background signal I …,nucleus . 5EU and Parp1 signals were significantly reduced at the DNA damage site marked by γH2AX. A one-sample signed rank test (*) was used to test the hypothesis H 0 : median=1, which gave P <0.001. For the higher rates of 10 and 50 Cpp, 5EU incorporation and Parp1 intensity significantly decreased to about 0.2 and 0.5, respectively. The Mann–Whitney rank sum test was used to test the difference of the medians for 10 or 50 Cpp compared with 1 Cpp, which gave + P <0.001 for 5EU and ++ P <0.05 for Parp1. Scale bars: 3 µm (for all cross-sections).

    Article Snippet: A fluorescent, tagRFP-labelled chromobody (ChromoTek) marks the endogenous Parp1 protein in these cells.

    Techniques: MANN-WHITNEY

    Analysis of single foci showing certain nucleolus hits on 5EU incorporation and Parp1 distribution. Frequency of single foci, showing certain nucleolus hits with dips in the 5EU and Parp1 signals. If the ratio was below 0.80, the area was defined as a dip. Error bars describe the 95% confidence Wilson score intervals of the binomial proportion.

    Journal: Journal of Cell Science

    Article Title: Local inhibition of rRNA transcription without nucleolar segregation after targeted ion irradiation of the nucleolus

    doi: 10.1242/jcs.232181

    Figure Lengend Snippet: Analysis of single foci showing certain nucleolus hits on 5EU incorporation and Parp1 distribution. Frequency of single foci, showing certain nucleolus hits with dips in the 5EU and Parp1 signals. If the ratio was below 0.80, the area was defined as a dip. Error bars describe the 95% confidence Wilson score intervals of the binomial proportion.

    Article Snippet: A fluorescent, tagRFP-labelled chromobody (ChromoTek) marks the endogenous Parp1 protein in these cells.

    Techniques: